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| Fall |
Winter |
Spring |
Summer |
| BIOL 6151 |
BIOL 6152 |
BIOL 6146 |
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| BIOL 6140 |
CHEM 6430 |
BIOL 4450 |
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| BIOL 4490 |
BIOL 4490 |
BIOL 4480 |
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Curriculum
The Certificate program is designed to be completed in a typical academic year of three quarters. All courses are presently offered during the day; however, plans for an evening program are under consideration.
Required Courses (32 units total)
Biology 4450 Cell Culture Techniques
This lecture/laboratory course covers introductory and advanced techniques in mammalian cell culture using attached and suspension cultures. Exercises include methods of cell enumeration, routine maintenance, growth curve establishment and analysis, primary culture, karyotyping, transformation assays, cytotoxicity measurements, cryopreservation, single cell cloning by cylinders and limiting dilution, in vitro differentiation systems, suspension cultures, transient and stable transfection.
Biology 4480 DNA Sequencing and Sequence Analysis
This laboratory course covers the sequencing of DNA and sequence analysis. Exercises include sequencing of PCR and plasmid templates, contig assembly and problem templates. All exercises include cycle sequencing reactions, preparation and running of sequencing gels on an ABI 377, editing and assembly of sequences, and sequence analysis.
Biology 4490 Introduction to Bioinformatics
This course is designed for biologists or computer scientists who are interested in a practical, and hands on approach to the field of computational molecular biology, especially the analysis of biological sequences and structures. Topics include: molecular biology databases, database searching, statistical techniques, genome annotation methods, multiple alignment, phylogenetic analysis, protein structural prediction, and microarray technology. The course format includes lecture followed by a workshop in the computer lab. Students need computer access outside of class time.
Biology 6140 Recombinant DNA Techniques
This is a laboratory intensive course presenting the fundamentals of recombinant DNA techniques. Laboratory exercises include transformation, plasmid and bacteriophage isolation and characterization, subcloning of gel purified fragments, and in vivo and in vitro analysis of recombinant DNA molecules, including PCR.
Two lecture hours a week will focus on an in-depth discussion of cloning vectors, subcloning, library construction and screening, and site-directed mutagenesis to complement the laboratory exercises. The lectures will also include a discussion of molecular cloning in eukaryotic organisms, including YAC construction and utilization, use of the yeast two-hybrid system and use of baculoviruses.
Biology 6146 PCR Technology
A lecture/laboratory course that covers the molecular mechanisms of the polymerase chain reaction and its widespread application to the research of gene structure and expression. Topics focus on experimental strategy and design, primer
design and synthesis, and PCR optimization. Laboratory exercises include targeted DNA amplification from plasmid and genomic templates, RT-PCR, quantitative RT-PCR, forensic applications, and PCR-product cloning.
Biology 6151 and 6152 Cell and Molecular Biology I, II
This is a two quarter series on contemporary cell and molecular biology. In addition to a traditional textbook and lecture format, extensive use is made of the current scientific literature. Lectures are supplemented with discussion groups and problem solving projects. Significant use is also made of online journals and databases. Topics in the first quarter include: current cell and molecular techniques, genetic analysis in molecular biology, molecular anatomy of the genome, DNA replication, repair and recombination, regulation of transcription, and RNA processing.
The second quarter (Biology 6152) topics include: protein synthesis and post-transcriptional control of gene expression, intracellular protein targeting and secretion, membrane structure and function, growth factors and signal transduction, cell cycle regulation, extracellular matrix biology, oncogenesis, cell differentiation, and apoptosis.
Chemistry 6430 Protein Chemistry Techniques
This course is designed to give the student a broad overview of protein chemistry techniques. The course stresses good laboratory notebook skills, calculations and experimental write-ups. Topics include: spectrophotometry, protein extraction, protein quantitation, gel filtration and ion exchange chromatography, affinity chromatography, HPLC, isoelectric focusing, peptide sequencing, recombinant protein isolation, SDS-PAGE, immunoblot analysis, and utilization of online protein databases.
Contact Info
For additional information, please e-mail cbaysdor@csuhayward.edu or call 510.885.3459.
California State University, Hayward
Department of Biological Sciences
Hayward, California 94542-3090 |
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